Reconstruction of a new Ionic temple incorporating the older temple in its foundation, eventually becoming associated with the Pan-lesbian Sanctuary of Messon.
Formation of the second Athenian alliance with Mytilene, Mithymna, Antissa and Eresos joining in. They take an active part in Alexander the Great’s campaign.
After the Antalkidean Peace, “autonomous” Lesbos is forced to recognize Persian rule. Tyrants are placed in the cities. Eresos sides with the Spartans.